期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 12, 页码 4566-4571出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1313368111
关键词
bacterial cytoskeleton; SOS response
资金
- European Research Council [243016]
- Marie Curie Intra-European Fellowship [PIEF-GA-2011-297918]
- Brazilian Swiss Joint Research Programme
- University of Lausanne
- Swiss National Science Foundation [3100A0_122541, 31003A_140758]
- Swiss National Science Foundation (SNF) [31003A_140758] Funding Source: Swiss National Science Foundation (SNF)
- European Research Council (ERC) [243016] Funding Source: European Research Council (ERC)
We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of Z-ring organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously unidentified period of rapid ring contraction in the final stages of the cell cycle. We also found that DNA damage resulted in production of high-density continuous Z-rings, which may obstruct cytokinesis. Our results provide a detailed quantitative picture of in vivo Z-ring organization.
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