期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 32, 页码 11762-11767出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1406102111
关键词
enzyme promiscuity; evolutionary innovation; molecular evolution; network evolution; phenotype microarray
资金
- Netherlands Organisation for Scientific Research Veni grant
- Lendulet Programme of the Hungarian Academy of Sciences
- Wellcome Trust
- European Research Council
- FP7 Initial Training Network METAFLUX (Metabolic Flux Analysis and Cancer)
- Hungarian Scientific Research Fund PD
- Hungarian Academy of Sciences Postdoctoral Fellowship Programme [SZ-039/2013]
- Tarsadalmi Megujulas Operativ Program Grant [4.2.4. A/2-11-1-2012-0001]
A central unresolved issue in evolutionary biology is how metabolic innovations emerge. Low-level enzymatic side activities are frequent and can potentially be recruited for new biochemical functions. However, the role of such underground reactions in adaptation toward novel environments has remained largely unknown and out of reach of computational predictions, not least because these issues demand analyses at the level of the entire metabolic network. Here, we provide a comprehensive computational model of the underground metabolism in Escherichia coli. Most underground reactions are not isolated and 45% of them can be fully wired into the existing network and form novel pathways that produce key precursors for cell growth. This observation allowed us to conduct an integrated genome-wide in silico and experimental survey to characterize the evolutionary potential of E. coli to adapt to hundreds of nutrient conditions. We revealed that underground reactions allow growth in new environments when their activity is increased. We estimate that at least similar to 20% of the underground reactions that can be connected to the existing network confer a fitness advantage under specific environments. Moreover, our results demonstrate that the genetic basis of evolutionary adaptations via underground metabolism is computationally predictable. The approach used here has potential for various application areas from bioengineering to medical genetics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据