期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 24, 页码 8877-8882出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1324105111
关键词
paired receptor; O-glycosylated protein; X-ray crystallography; immune regulation
资金
- Platform for Drug Discovery, Informatics, and Structural Life Science
- Ministry of Education, Culture, Sports, Science and Technology
- Ministry of Health, Labor and Welfare of Japan
- Japan Society for the Promotion of Science Research Fellowships for Young Scientists
- Grants-in-Aid for Scientific Research [23570139, 26117714, 24659223, 24115005] Funding Source: KAKEN
Paired Ig-like type 2 receptor a (PILR alpha) recognizes a wide range of O-glycosylated mucin and related proteins to regulate broad immune responses. However, the molecular characteristics of these recognitions are largely unknown. Here we show that sialylated O-linked sugar T antigen (sTn) and its attached peptide region are both required for ligand recognition by PILR alpha. Furthermore, we determined the crystal structures of PILR alpha and its complex with an sTn and its attached peptide region. The structures show that PILR alpha exhibits large conformational change to recognize simultaneously both the sTn O-glycan and the compact peptide structure constrained by proline residues. Binding and functional assays support this binding mode. These findings provide significant insight into the binding motif and molecular mechanism (which is distinct from sugar-recognition receptors) by which O-glycosylated mucin proteins with sTn modifications are recognized in the immune system as well as during viral entry.
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