期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 11, 页码 4025-4030出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1314485111
关键词
fruit fly; ribosome; dioxygenase; proline; tranlational stress
资金
- Wellcome Trust [WT087675MA]
- Biotechnology and Biological Sciences Research Council UK
- Agencia Nacional de Promocion Cientifica y Tecnologica [0090]
- BBSRC [BB/L004275/1, BB/J003018/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L004275/1, BB/J003018/1] Funding Source: researchfish
Genome sequences predict the presence of many 2-oxoglutarate (2OG)-dependent oxygenases of unknown biochemical and biological functions in Drosophila. Ribosomal protein hydroxylation is emerging as an important 2OG oxygenase catalyzed pathway, but its biological functions are unclear. We report investigations on the function of Sudestada1 (Sud1), a Drosophila ribosomal oxygenase. As with its human and yeast homologs, OGFOD1 and Tpa1p, respectively, we identified Sud1 to catalyze prolyl-hydroxylation of the small ribosomal subunit protein RPS23. Like OGFOD1, Sud1 catalyzes a single prolyl-hydroxylation of RPS23 in contrast to yeast Tpa1p, where Pro-64 dihydroxylation is observed. RNAi-mediated Sud1 knockdown hinders normal growth in different Drosophila tissues. Growth impairment originates from both reduction of cell size and diminution of the number of cells and correlates with impaired translation efficiency and activation of the unfolded protein response in the endoplasmic reticulum. This is accompanied by phosphorylation of eIF2 alpha and concomitant formation of stress granules, as well as promotion of autophagy and apoptosis. These observations, together with those on enzyme homologs described in the companion articles, reveal conserved biochemical and biological roles for a widely distributed ribosomal oxygenase.
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