期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 5, 页码 1813-1818出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1323931111
关键词
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资金
- Ministry of Science and Technology [2009CB918801]
- National Natural Science Foundation [31021002, 31130002]
- Beijing Municipal Commissions of Education and Science and Technology
Vitamin C, also known as ascorbate, is required in numerous essential metabolic reactions in eukaryotes. The eukaryotic ascorbate-dependent oxidoreductase cytochrome b(561) (Cyt b(561)), a family of highly conserved transmembrane enzymes, plays an important role in ascorbate recycling and iron absorption. Although Cyt b(561) was identified four decades ago, its atomic structure and functional mechanism remain largely unknown. Here, we report the high-resolution crystal structures of cytochrome b(561) from Arabidopsis thaliana in both substrate-free and substrate-bound states. Cyt b(561) forms a homodimer, with each protomer consisting of six transmembrane helices and two heme groups. The negatively charged substrate ascorbate, or monodehydroascorbate, is enclosed in a positively charged pocket on either side of the membrane. Two highly conserved amino acids, Lys(81) and His(106), play an essential role in substrate recognition and catalysis. Our structural and biochemical analyses allow the proposition of a general electron transfer mechanism for members of the Cyt b(561) family.
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