期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 21, 页码 7606-7611出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1321232111
关键词
thiol redox; hydrogen sulfide; electrophilic signaling; polysulfidomics
资金
- Japan Science and Technology Agency
- Program for the Strategic Research Foundation at Private Universities
- Biobank Japan Project
- Ministry of Education, Sciences, Sports, and Technology, Japan
- National Institutes of Health [R01HL116571, HL106598]
- National Science Foundation [CHE-0844931, CHE-1148641]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0844931] Funding Source: National Science Foundation
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1148641] Funding Source: National Science Foundation
- Grants-in-Aid for Scientific Research [20117005, 26670131, 24390075, 25870549, 24590527, 25430069, 24790263, 26670150, 24390082] Funding Source: KAKEN
Using methodology developed herein, it is found that reactive persulfides and polysulfides are formed endogenously from both small molecule species and proteins in high amounts in mammalian cells and tissues. These reactive sulfur species were biosynthesized by two major sulfurtransferases: cystathionine beta-synthase and cystathionine gamma-lyase. Quantitation of these species indicates that high concentrations of glutathione persulfide (perhydropersulfide >100 mu M) and other cysteine persulfide and polysulfide derivatives in peptides/proteins were endogenously produced and maintained in the plasma, cells, and tissues of mammals (rodent and human). It is expected that persulfides are especially nucleophilic and reducing. This view was found to be the case, because they quickly react with H2O2 and a recently described biologically generated electrophile 8-nitroguanosine 3',5'-cyclic monophosphate. These results indicate that persulfides are potentially important signaling/effector species, and because H2S can be generated from persulfide degradation, much of the reported biological activity associated with H2S may actually be that of persulfides. That is, H2S may act primarily as a marker for the biologically active of persulfide species.
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