期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 41, 页码 14794-14799出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1410124111
关键词
TNFalpha; NF-kappaB; metalloproteinase; posttranslational modification
资金
- Agence Nationale pour la Recherche
- Association pour la Recherche sur le Cancer
- Institut National du Cancer
- Universite Paris Descartes
- ANR
- INCa
TNF alpha is a potent cytokine that plays a critical role in numerous cellular processes, particularly immune and inflammatory responses, programmed cell death, angiogenesis, and cell migration. Thus, understanding the molecular mechanisms that mediate TNF alpha-induced cellular responses is a crucial issue. It is generally accepted that global DNA binding activity of the NF-kappa B avian reticuloendotheliosis viral (v-rel) oncogene related B (RelB) subunit is not induced upon TNFa treatment in fibroblasts, despite its TNF alpha-induced nuclear accumulation. Here, we demonstrate that RelB plays a critical role in promoting fibroblast migration upon prolonged TNF alpha treatment. We identified the two kinases I kappa B kinase a (IKK alpha) and I kappa B kinase beta (IKK beta) as RelB interacting partners whose activation by TNF alpha promotes RelB phosphorylation at serine 472. Once phosphorylated on serine 472, nuclear RelB dissociates from its interaction with the inhibitory protein I kappa B alpha and binds to the promoter of critical migration-associated genes, such as the matrix metallopeptidase 3 (MMP3). Further, we show that RelB serine 472 phosphorylation status controls MMP3 expression and promigration activity downstream of TNF receptors. Our findings provide new insights into the regulation of RelB activity and reveal a novel link between selective NF-kappa B target gene expression and cellular response in response to TNF alpha.
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