期刊
ONCOLOGY LETTERS
卷 10, 期 5, 页码 3318-3322出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2015.3686
关键词
glioma; BMI-1; copy number; overexpression
类别
资金
- Council of Scientific and Industrial Research, New Delhi
- Department of Science and Technology-Science and Engineering Research Board, Government of India [SR/SO/HS-233/2012]
Malignant gliomas are neoplasms of the brain that are associated with a poor prognosis. The B-cell-specific Moloney murine leukemia virus integration site 1 (BMI-1) gene is one of the major cancer stem cell factors responsible for treatment failure in glioma. In the present study, the DNA-RNA-protein alterations in the BMI-1 gene were assessed in 50 glioma samples. Copy number variations in the BMI-1 gene were analyzed using SYBR (R) Green quantitative polymerase chain reaction. Gene expression analysis was performed using a Taqman assay and protein quantitation was performed using western blotting. A comparative Ct analysis showed the absence of copy number variations in all glioma samples. BMI-1 mRNA expression was found to be overexpressed in 36 out of 50 samples ( 72.0%), and 37 out of 50 samples showed overexpression (74.0%) of BMI-1 protein; this was statistically significant when compared with non-glioma tissues. It was observed that the protein and RNA expression in glioma were concordant. In this study on the BMI-1 gene, transcription and translation in glioma were observed and BMI-1 overexpression was found to be a common phenomenon.
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