期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 110, 期 26, 页码 10866-10871出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1301647110
关键词
phytohormone analyses; chromatin immunoprecipitation; transcriptomic analyses
资金
- Biotechnology and Biological Sciences Research Council [BB/E000541/1, BB/J000949/1, BB/F005296/1, BB/F005237/1]
- Garfield Weston Foundation
- BBSRC [BB/E000541/1, BB/J00216X/1, BB/F005237/1, BB/J000949/1, BB/F005296/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J00216X/1, BB/F005296/1, BB/F005237/1, BB/E000541/1, BB/J000949/1] Funding Source: researchfish
Freshly matured seeds exhibit primary dormancy, which prevents germination until environmental conditions are favorable. The establishment of dormancy occurs during seed development and involves both genetic and environmental factors that impact on the ratio of two antagonistic phytohormones: abscisic acid (ABA), which promotes dormancy, and gibberellic acid, which promotes germination. Although our understanding of dormancy breakage in mature seeds is well advanced, relatively little is known about the mechanisms involved in establishing dormancy during seed maturation. We previously showed that the SPATULA (SPT) transcription factor plays a key role in regulating seed germination. Here we investigate its role during seed development and find that, surprisingly, it has opposite roles in setting dormancy in Landsberg erecta and Columbia Arabidopsis ecotypes. We also find that SPT regulates expression of five transcription factor encoding genes: ABA-INSENSITIVE4 (ABI4) and ABI5, which mediate ABA signaling; REPRESSOR-OF-GA (RGA) and RGA-LIKE3 involved in gibberellic acid signaling; and MOTHER-OF-FT-AND-TFL1 (MFT) that we show here promotes Arabidopsis seed dormancy. Although ABI4, RGA, and MFT are repressed by SPT, ABI5 and RGL3 are induced. Furthermore, we show that RGA, MFT, and ABI5 are direct targets of SPT in vivo. We present a model in which SPT drives two antagonistic dormancy- repressing and dormancy-promoting routes that operate simultaneously in freshly matured seeds. Each of these routes has different impacts and this in turn explains the opposite effect of SPT on seed dormancy of the two ecotypes analyzed here.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据