期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 110, 期 24, 页码 10028-10033出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1300403110
关键词
cell signaling; root development; proteasome; two-component system
资金
- US Department of Agriculture, Agriculture Food and Research Initiative/National Research Institute [2007-35304-18323]
- National Science Foundation [IOS-0618286]
- Human Frontier Science Program [LT000757/2009-L]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1022053] Funding Source: National Science Foundation
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1039423] Funding Source: National Science Foundation
Cytokinins are plant hormones that play critical roles in growth and development. In Arabidopsis, the transcriptional response to cytokinin is regulated by action of type-B Arabidopsis response regulators (ARRs). Although central elements in the cytokinin signal transduction pathway have been identified, mechanisms controlling output remain to be elucidated. Here we demonstrate that a family of F-box proteins, called the KISS ME DEADLY (KMD) family, targets type-B ARR proteins for degradation. KMD proteins form an S-PHASE KINASE-ASSOCIATED PROTEIN1 (SKP1)/Cullin/F-box protein (SCF) E3 ubiquitin ligase complex and directly interact with type-B ARR proteins. Loss-of-function KMD mutants stabilize type-B ARRs and exhibit an enhanced cytokinin response. In contrast, plants with elevated KMD expression destabilize type-B ARR proteins leading to cytokinin insensitivity. Our results support a model in which an SCFKMD complex negatively regulates cytokinin responses by controlling levels of a key family of transcription factors.
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