期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 110, 期 27, 页码 10976-10981出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1309690110
关键词
cross-beta structure; nanomechanics; microcantilever
资金
- National Science Foundation [DMR-0964886]
- Air Force Office of Scientific Research [FA9550-11-1-0055]
Amyloid is an important class of proteinaceous material because of its close association with protein misfolding disorders such as Alzheimer's disease and type II diabetes. Although the degree of stiffness of amyloid is critical to the understanding of its pathological and biological functions, current estimates of the rigidity of these beta-sheet-rich protein aggregates range from soft (10(8) Pa) to hard (10(10) Pa) depending on the method used. Here, we use time-resolved 4D EM to directly and noninvasively measure the oscillatory dynamics of freestanding, self-supporting amyloid beams and their rigidity. The dynamics of a single structure, not an ensemble, were visualized in space and time by imaging in the microscope an amyloid-dye cocrystal that, upon excitation, converts light into mechanical work. From the oscillatory motion, together with tomographic reconstructions of three studied amyloid beams, we determined the Young modulus of these highly ordered, hydrogen-bonded beta-sheet structures. We find that amyloid materials are very stiff (10(9) Pa). The potential biological relevance of the deposition of such a highly rigid biomaterial in vivo are discussed.
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