期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 110, 期 26, 页码 10854-10859出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1302592110
关键词
gene expression; hypoxia
资金
- Office of Science of the US Department of Energy [DE-AC02-05CH11231]
- Deutsche Forschungsgemeinschaft [He 5790/1-1]
- Deutsches Zentrum fur Luft- und Raumfahrt
- National Institutes of Health [GM42143]
Hemoglobins are recognized today as a diverse family of proteins present in all kingdoms of life and performing multiple reactions beyond O-2 chemistry. The physiological roles of most hemoglobins remain elusive. Here, we show that a 2-on-2 (truncated) hemoglobin, termed THB8, is required for hypoxic growth and the expression of anaerobic genes in Chlamydomonas reinhardtii. THB8 is 1 of 12 2-on-2 hemoglobins in this species. It belongs to a subclass within the 2-on-2 hemoglobin class I family whose members feature a remarkable variety of domain arrangements and lengths. Posttranscriptional silencing of the THB8 gene results in the mis-regulation of several genes and a growth defect under hypoxic conditions. The latter is intensified in the presence of an NO scavenger, which also impairs growth of wild-type cells. As recombinant THB8 furthermore reacts with NO, the results of this study indicate that THB8 is part of an NO-dependent signaling pathway.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据