期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 110, 期 30, 页码 12289-12294出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1300781110
关键词
translation regulation; SILAC; systems biology
资金
- Scottish Institute for Cell Signalling
- College of Life Sciences, University of Dundee, Innovation Pipeline for Translational Science [LUPS/ERDF/2008/2/1/0429]
- Ambizione grant of the Swiss National Science Foundation
- College of Life Sciences Bursary at the University of Dundee
- Max Planck Society
- Deutsche Forschungsgemeinschaft
- Promedica Foundation
- MRC [G1100713] Funding Source: UKRI
- Medical Research Council [G1100713] Funding Source: researchfish
tRNA modifications are crucial to ensure translation efficiency and fidelity. In eukaryotes, the URM1 and ELP pathways increase cellular resistance to various stress conditions, such as nutrient starvation and oxidative agents, by promoting thiolation and methoxycarbonylmethylation, respectively, of the wobble uridine of cytoplasmic tRNA(Lys)(UUU) (tK(UUU)), tRNA(Gln)(UUG) (tQ(UUG)), and tRNA(Glu)(UUC) (tE(UUC)). Although in vitro experiments have implicated these tRNA modifications in modulating wobbling capacity and translation efficiency, their exact in vivo biological roles remain largely unexplored. Using a combination of quantitative proteomics and codon-specific translation reporters, we find that translation of a specific gene subset enriched for AAA, CAA, and GAA codons is impaired in the absence of URM1- and ELP-dependent tRNA modifications. Moreover, in vitro experiments using native tRNAs demonstrate that both modifications enhance binding of tK(UUU) to the ribosomal A-site. Taken together, our data suggest that tRNA thiolation and methoxycarbonylmethylation regulate translation of genes with specific codon content.
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