期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 29, 页码 11630-11635出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1204718109
关键词
photonics; microfluidics; instrumentation; high-throughput screening; medical diagnostics
资金
- U.S. Congressionally Directed Medical Research Programs [W81XWH1010519]
- Microsystems Technology Office in the U.S. Defense Advanced Research Projects Agency
- National Institutes of Health (NIH)
- Caltech-UCLA Joint Center for Translational Medicine
- Burroughs Wellcome Fund
- German Research Foundation
- Natural Sciences and Engineering Research Council of Canada
- NIH [CA-16042, AI-28697]
- JCCC
- UCLA AIDS Institute
- David Geffen School of Medicine at UCLA
- U.S. Department of Defense (DOD) [W81XWH1010519] Funding Source: U.S. Department of Defense (DOD)
Optical microscopy is one of the most widely used diagnostic methods in scientific, industrial, and biomedical applications. However, while useful for detailed examination of a small number (<10,000) of microscopic entities, conventional optical microscopy is incapable of statistically relevant screening of large populations (>100,000,000) with high precision due to its low throughput and limited digital memory size. We present an automated flow-through single-particle optical microscope that overcomes this limitation by performing sensitive blur-free image acquisition and nonstop real-time image-recording and classification of microparticles during high-speed flow. This is made possible by integrating ultrafast optical imaging technology, self-focusing microfluidic technology, optoelectronic communication technology, and information technology. To show the system's utility, we demonstrate high-throughput image-based screening of budding yeast and rare breast cancer cells in blood with an unprecedented throughput of 100,000 particles/s and a record false positive rate of one in a million.
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