期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 29, 页码 11794-11799出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1116584109
关键词
transcription factors; transgenic mice
资金
- Deutsche Forschungsgemeinschaft [SFB/TR19]
Inflammation is a major factor in heart disease. I kappa B kinase (IKK) and its downstream target NF-kappa B are regulators of inflammation and are activated in cardiac disorders, but their precise contributions and targets are unclear. We analyzed IKK/NF-kappa B function in the heart by a gain-of-function approach, generating an inducible transgenic mouse model with cardiomyocyte-specific expression of constitutively active IKK2. In adult animals, IKK2 activation led to inflammatory dilated cardiomyopathy and heart failure. Transgenic hearts showed infiltrationwith CD11b(+) cells, fibrosis, fetal reprogramming, and atrophy of myocytes with strong constitutively active IKK2 expression. Upon transgene inactivation, the disease was reversible even at an advanced stage. IKK-induced cardiomyopathywas dependent on NF-kappa B activation, as in vivo expression of I kappa B alpha superrepressor, an inhibitor of NF-kappa B, prevented the development of disease. Gene expression and proteomic analyses revealed enhanced expression of inflammatory cytokines, and an IFN type I signature with activation of the IFN-stimulated gene 15 (ISG15) pathway. In that respect, IKK-induced cardiomyopathy resembled Coxsackievirus-induced myocarditis, during which the NF-kappa B and ISG15 pathways were also activated. Vice versa, in cardiomyocytes lacking the regulatory subunit of IKK (IKK gamma/NEMO), the induction of ISG15 was attenuated. We conclude that IKK/NF-kappa B activation in cardiomyocytes is sufficient to cause cardiomyopathy and heart failure by inducing an excessive inflammatory response and myocyte atrophy.
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