期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 15, 页码 E905-E913出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1119172109
关键词
-
资金
- Graduate School of Science of the University of Groningen
- Agency of the Academy of Sciences of the Czech Republic [IAA600200801]
- Czech Science Foundation [P302/12/0256, P207/12/1568]
- Ministry of Education, Youth and Sports of the Czech Republic [LH 12055]
- Institutional Research Concept [AV0Z50200510]
- Italian Ministry of Instruction, University and Research (MIUR)
- University of Cagliary
- European Union
- Netherlands Organization for Scientific Research, Earth and Life Sciences (NWO-ALW)
- NWO-ALW
- Netherlands Organization for Health Research and Development (NWO-ZonMW)
How the human pathogen Streptococcus pneumoniae coordinates cell-wall synthesis during growth and division to achieve its characteristic oval shape is poorly understood. The conserved eukaryotic-type Ser/Thr kinase of S. pneumoniae, StkP, previously was reported to phosphorylate the cell-division protein DivIVA. Consistent with a role in cell division, GFP-StkP and its cognate phosphatase, GFP-PhpP, both localize to the division site. StkP localization depends on its penicillin-binding protein and Ser/Thr-associated domains that likely sense uncross-linked peptidoglycan, because StkP and PhpP delocalize in the presence of antibiotics that target the latest stages of cell-wall biosynthesis and in cells that have stopped dividing. Time-lapse microscopy shows that StkP displays an intermediate timing of recruitment to midcell: StkP arrives shortly after FtsA but before DivIVA. Furthermore, StkP remains at midcell longer than FtsA, until division is complete. Cells mutated for stkP are perturbed in cell-wall synthesis and display elongated morphologies with multiple, often unconstricted, FtsA and DivIVA rings. The data show that StkP plays an important role in regulating cell-wall synthesis and controls correct septum progression and closure. Overall, our results indicate that StkP signals information about the cell-wall status to key cell-division proteins and in this way acts as a regulator of cell division.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据