期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 110, 期 2, 页码 791-796出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1211447110
关键词
phenotyping; imaging; ethology; nematode
资金
- National Institutes of Health (NIH) Chemical Genomics Center Office of Research Infrastructure [P40 OD010440]
- Medical Research Council [MC-A022-5PB91]
- NIH [DA018341]
- Human Frontier Science Program
- Medical Research Council [MC_U105185857] Funding Source: researchfish
- MRC [MC_U105185857] Funding Source: UKRI
Visible phenotypes based on locomotion and posture have played a critical role in understanding the molecular basis of behavior and development in Caenorhabditis elegans and other model organisms. However, it is not known whether these human-defined features capture the most important aspects of behavior for phenotypic comparison or whether they are sufficient to discover new behaviors. Here we show that four basic shapes, or eigenworms, previously described for wild-type worms, also capture mutant shapes, and that this representation can be used to build a dictionary of repetitive behavioral motifs in an unbiased way. By measuring the distance between each individual's behavior and the elements in the motif dictionary, we create a fingerprint that can be used to compare mutants to wild type and to each other. This analysis has revealed phenotypes not previously detected by real-time observation and has allowed clustering of mutants into related groups. Behavioral motifs provide a compact and intuitive representation of behavioral phenotypes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据