期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 110, 期 1, 页码 318-323出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1212708110
关键词
thick filaments 3D structure; human heart muscle; electron microscopy; image processing
资金
- British Heart Foundation [FS/07/017/22951]
- National Institutes of Health (NIH) [5SC1HL96017]
- Research Centers in Minority Institutions Award from the National Center for Research Resources, NIH [G12RR-03051]
- British Heart Foundation [PG/07/076/23480, FS/07/017/22951] Funding Source: researchfish
Of all the myosin filaments in muscle, the most important in terms of human health, and so far the least studied, are those in the human heart. Here we report a 3D single-particle analysis of electron micrograph images of negatively stained myosin filaments isolated from human cardiac muscle in the normal (undiseased) relaxed state. The resulting 28-angstrom resolution 3D reconstruction shows axial and azimuthal (no radial) myosin head perturbations within the 429-angstrom axial repeat, with rotations between successive 132 angstrom-, 148 angstrom-, and 149 angstrom-spaced crowns of heads close to 60 degrees, 35 degrees, and 25 degrees (all would be 40 degrees in an unperturbed three-stranded helix). We have defined the myosin head atomic arrangements within the three crown levels and have modeled the organization of myosin subfragment 2 and the possible locations of the 39 angstrom-spaced domains of titin and the cardiac isoform of myosin-binding protein-C on the surface of the myosin filament backbone. Best fits were obtained with head conformations on all crowns close to the structure of the two-headed myosin molecule of vertebrate chicken smooth muscle in the dephosphorylated relaxed state. Individual crowns show differences in head-pair tilts and subfragment 2 orientations, which, together with the observed perturbations, result in different intercrown head interactions, including one not reported before. Analysis of the interactions between the myosin heads, the cardiac isoform of myosin-binding protein-C, and titin will aid in understanding of the structural effects of mutations in these proteins known to be associated with human cardiomyopathies.
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