期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 38, 页码 15425-15430出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1208031109
关键词
crystallography; pattern recognition; proteolysis; structural biology
资金
- Danscatt
- Lundbeck Foundation
- Lundbeck Foundation Nanomedicine Centre for Individualized Management of Tissue Damage and Regeneration
- Novo-Nordisk Foundation
- Hungarian Scientific Research Fund (OTKA) [NK77978]
- National Development Agency [KMOP-1.1.2-07/1-2008-0003]
- Janos Bolyai Foundation
An essential aspect of innate immunity is recognition of molecular patterns on the surface of pathogens or altered self through the lectin and classical pathways, two of the three well-established activation pathways of the complement system. This recognition causes activation of the MASP-2 or the C1s serine proteases followed by cleavage of the protein C4. Here we present the crystal structures of the 203-kDa human C4 and the 245-kDa C4.MASP-2 substrate.enzyme complex. When C4 binds to MASP-2, substantial conformational changes in C4 are induced, and its scissile bond region becomes ordered and inserted into the protease catalytic site in a manner canonical to serine proteases. In MASP-2, an exosite located within the CCP domains recognizes the C4 C345C domain 60 angstrom from the scissile bond. Mutations in C4 and MASP-2 residues at the C345C-CCP interface inhibit the intermolecular interaction and C4 cleavage. The possible assembly of the huge in vivo enzyme-substrate complex consisting of glycan-bound mannan-binding lectin, MASP-2, and C4 is discussed. Our own and prior functional data suggest that C1s in the classical pathway of complement activated by, e. g., antigen-antibody complexes, also recognizes the C4 C345C domain through a CCP exosite. Our results provide a unified structural framework for understanding the early and essential step of C4 cleavage in the elimination of pathogens and altered self through two major pathways of complement activation.
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