期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 8, 页码 2919-2924出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1111662109
关键词
fluorescence microscopy; multiphoton imaging; multiphoton microscopy; three-dimensional microscopy
资金
- Engineering and Physical Sciences Research Council (EPSCR) [EP/H018565/1]
- Biotechnology and Biological Sciences Research Council
- Wellcome Trust
- EPSRC [EP/F042647/1]
- BBSRC [BB/F016042/1] Funding Source: UKRI
- EPSRC [EP/H018565/1, EP/F042647/1, EP/E055818/1, EP/F048009/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F016042/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/E055818/1, EP/F042647/1, EP/H018565/1] Funding Source: researchfish
Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.
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