期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 12, 页码 4668-4673出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1201498109
关键词
environmental sensing; systems biology
资金
- Biotechnology and Biological Sciences Research Council
- Engineering and Physical Sciences Research Council
- European Community [PIEF-GA-2008-220506]
- National Institutes of Health and Howard Hughes Medical Institute
- Research Foundation Flanders
- Belgian Scientific policy
- Human Frontier Science Program Organization
- Agence National de la Recherche
- BBSRC [BB/D019613/1, BB/J009717/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J009717/1, REI20541, BB/D019613/1] Funding Source: researchfish
Gravity profoundly influences plant growth and development. Plants respond to changes in orientation by using gravitropic responses to modify their growth. Cholodny and Went hypothesized over 80 years ago that plants bend in response to a gravity stimulus by generating a lateral gradient of a growth regulator at an organ's apex, later found to be auxin. Auxin regulates root growth by targeting Aux/IAA repressor proteins for degradation. We used an Aux/IAA-based reporter, domain II (DII)-VENUS, in conjunction with a mathematical model to quantify auxin redistribution following a gravity stimulus. Our multidisciplinary approach revealed that auxin is rapidly redistributed to the lower side of the root within minutes of a 90 degrees gravity stimulus. Unexpectedly, auxin asymmetry was rapidly lost as bending root tips reached an angle of 40 degrees to the horizontal. We hypothesize roots use a tipping point mechanism that operates to reverse the asymmetric auxin flow at the midpoint of root bending. These mechanistic insights illustrate the scientific value of developing quantitative reporters such as DII-VENUS in conjunction with parameterized mathematical models to provide high-resolution kinetics of hormone redistribution.
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