期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 12, 页码 4377-4382出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1201086109
关键词
nanopatterning; polymer pen lithography; focal adhesions; osteogenesis; stem cell differentiation
资金
- Air Force Office of Scientific Research (AFOSR) [FA9550-08-1-0124]
- SPAWAR Systems Center [22550, N66001-08-1-2044]
- National Cancer Institute [U54CA151880]
- National Science Foundation (NSF) Nanoscale Science and Engineering Initiative [EEC-0647560]
- Defense Advanced Research Projects Agency (DARPA)
- Engineering and Physical Sciences Research Council (EPSRC) [EP/F042590/1]
- EPSRC [EP/F042590/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/F042590/1] Funding Source: researchfish
We report the development of a powerful analytical method that utilizes a tilted elastomeric pyramidal pen array in the context of a scanning probe lithography experiment to rapidly prepare libraries having as many as 25 million features over large areas with a range of feature sizes from the nano- to microscale. This technique can be used to probe important chemical and biological processes, opening up the field of nanocombinatorics. In a proof-of-concept investigation of mesenchymal stem cell (MSC) differentiation, combinatorial patterns first enabled a rapid and systematic screening of MSC adhesion, as a function of feature size, while uniform patterns were used to study differentiation with statistically significant sample sizes. Without media containing osteogenic-inducing chemical cues, cells cultured on nanopatterned fibronectin substrates direct MSC differentiation towards osteogenic fates when compared to nonpatterned fibronectin substrates. This powerful and versatile approach enables studies of many systems spanning biology, chemistry, and engineering areas.
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