期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 26, 页码 10214-10217出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1200817109
关键词
BLBP; DOFL; high throughput screening; mass spectrometry; radial glia
资金
- A*STAR (Agency for Science, Technology and Research, Singapore) Biomedical Research Council
- Singapore Ministry of Education Academic Research Fund Tier 2 [MOE2010-T2-1-025]
Fluorescent small molecules have become indispensable tools for biomedical research along with the rapidly developing optical imaging technology. We report here a neural stem cell specific boron-dipyrromethane (BODIPY) derivative compound of designation red 3 (CDr3), developed through a high throughput/content screening of in-house generated diversity oriented fluorescence library in stem cells at different developmental stages. This novel compound specifically detects living neural stem cells of both human and mouse origin. Furthermore, we identified its binding target by proteomic analysis as fatty acid binding protein 7 (FABP7), also known as brain lipid binding protein) which is highly expressed in neural stem cells and localized in the cytoplasm. CDr3 will be a valuable chemical tool in the study and applications of neural stem cells.
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