期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 26, 页码 10498-10503出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1202588109
关键词
innate immunity; complement system; directed evolution; phage display; canonical inhibitor
资金
- Anyos Jedlik Grant [NKFP_07_1-MASPOK07]
- Hungarian Scientific Research Fund (OTKA) Grants [NK77978, NK100769, NK100834, K68408, NK81950]
- National Development Agency Grant [KMOP-1.1.2-07/1-2008-0003]
- European Union
- European Social Fund (TAMOP) Grant [4.2.1./B-09/KMR-2010-0003]
The lectin pathway of complement activation is an important component of the innate immune defense. The initiation complexes of the lectin pathway consist of a recognition molecule and associated serine proteases. Until now the autoactivating mannose-binding lectin-associated serine protease (MASP)-2 has been considered the autonomous initiator of the proteolytic cascade. The role of the much more abundant MASP-1 protease was controversial. Using unique, monospecific inhibitors against MASP-1 and MASP-2, we corrected the mechanism of lectin-pathway activation. In normal human serum, MASP-2 activation strictly depends on MASP-1. MASP-1 activates MASP-2 and, moreover, inhibition of MASP-1 prevents autoactivation of MASP-2. Furthermore we demonstrated that MASP-1 produces 60% of C2a responsible for C3 convertase formation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据