期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 18, 页码 6969-6974出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1201204109
关键词
excitation-transcription coupling; transcription
资金
- Medical Research Council
- Medical Research Council [G1000813] Funding Source: researchfish
- MRC [G1000813] Funding Source: UKRI
Stimulation of cells with physiological concentrations of calcium-mobilizing agonists often results in the generation of repetitive cytoplasmic Ca2+ oscillations. Although oscillations arise from regenerative Ca2+ release, they are sustained by store-operated Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. Here, we show that following stimulation of cysteinyl leukotriene type I receptors in rat basophilic leukemia (RBL)-1 cells, large amplitude Ca2+ oscillations, CRAC channel activity, and downstream Ca2+-dependent nuclear factor of activated T cells (NFAT)-driven gene expression are all exclusively maintained by the endoplasmic reticulum Ca2+ sensor stromal interaction molecule (STIM) 1. However, stimulation of tyrosine kinase-coupled FC epsilon RI receptors evoked Ca2+ oscillations and NFAT-dependent gene expression through recruitment of both STIM2 and STIM1. We conclude that different agonists activate different STIM proteins to sustain Ca2+ signals and downstream responses.
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