期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 21, 页码 8115-8120出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1204759109
关键词
molecular motors; single-molecule; strand displacement; replicative DNA polymerase
资金
- [BFU2011-29038-CO2-1]
- [CSD2007-00010]
- [BFU2010-15703/BMC]
- [BFU2008-00215/BMC]
- [CSD2007-00015]
- [RYC2007-01765]
- [S2009MAT-1507]
- [FIS2010-17440]
- [GR35/10-A-920GR35/10-A-911]
Duplication of double-stranded DNA (dsDNA) requires a fine-tuned coordination between the DNA replication and unwinding reactions. Using optical tweezers, we probed the coupling dynamics between these two activities when they are simultaneously carried out by individual Phi29 DNA polymerase molecules replicating a dsDNA hairpin. We used the wild-type and an unwinding deficient polymerase variant and found that mechanical tension applied on the DNA and the DNA sequence modulate in different ways the replication, unwinding rates, and pause kinetics of each polymerase. However, incorporation of pause kinetics in a model to quantify the unwinding reaction reveals that both polymerases destabilize the fork with the same active mechanism and offers insights into the topological strategies that could be used by the Phi29 DNA polymerase and other DNA replication systems to couple unwinding and replication reactions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据