期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 43, 页码 17693-17698出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1212786109
关键词
cerebellum; P/Q-type calcium channel; inherited ataxia
资金
- Japan Society for the Promotion of Science
- Ministry of Education, Science, and Culture of Japan
- Core Research for Evolutional Science and Technology (CREST)
- Japan Science and Technology Agency
- Comprehensive Brain Science Network
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [23790238, 22590924, 22659335] Funding Source: KAKEN
Spinocerebellar ataxia type 6 (SCA6) is a neurodegenerative disease caused by the expansion of a polyglutamine tract in the Ca(v)2.1 voltage-gated calcium channel. To elucidate how the expanded polyglutamine tract in this plasma membrane protein causes the disease, we created a unique knockin mouse model that modestly overexpressed the mutant transcripts under the control of an endogenous promoter (MPI-118Q). MPI-118Q mice faithfully recapitulated many features of SCA6, including selective Purkinje cell degeneration. Surprisingly, analysis of inclusion formation in the mutant Purkinje cells indicated the lysosomal localization of accumulated mutant Ca(v)2.1 channels in the absence of autophagic response. The lack of cathepsin B, a major lysosomal cysteine proteinase, exacerbated the loss of Purkinje cells and was accompanied by an acceleration of inclusion formation in this model. Thus, the pathogenic mechanism of SCA6 involves the endolysosomal degradation pathway, and unique pathological features of this model further illustrate the pivotal role of protein context in the pathogenesis of polyglutamine diseases.
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