期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 29, 页码 11705-11710出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1121583109
关键词
cytoskeleton; self-organization; symmetry breaking
资金
- Centre National de la Recherche Scientifique
- Association pour la Recherche sur le Cancer [SFI20101201426]
- Agence Nationale de la Recherche (ANR) [ANR-08-PNANO-050]
- Ligue Nationale Contre le Cancer (LNCC)
- Ville de Paris Emergence
In vivo, F-actin flows are observed at different cell life stages and participate in various developmental processes during asymmetric divisions in vertebrate oocytes, cell migration, or wound healing. Here, we show that confinement has a dramatic effect on F-actin spatiotemporal organization. We reconstitute in vitro the spontaneous generation of F-actin flow using Xenopus meiotic extracts artificially confined within a geometry mimicking the cell boundary. Perturbations of actin polymerization kinetics or F-actin nucleation sites strongly modify the network flow dynamics. A combination of quantitative image analysis and biochemical perturbations shows that both spatial localization of F-actin nucleators and actin turnover play a decisive role in generating flow. Interestingly, our in vitro assay recapitulates several symmetry-breaking processes observed in oocytes and early embryonic cells.
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