期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 3, 页码 799-804出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1119057109
关键词
single molecule FRET; small angle X-ray scattering; worm-like chain; ion-nucleic acid interactions
资金
- National Institutes of Health (NIH) [R01-GM085062, T32-GM008267]
- Cornell Nanobiotechnology Center
- National Science Foundation (NSF)
- NIH/National Institute of General Medical Sciences under NSF [DMR-0225180]
Dynamic RNA molecules carry out essential processes in the cell including translation and splicing. Base-pair interactions stabilize RNA into relatively rigid structures, while flexible non-base-paired regions allow RNA to undergo conformational changes required for function. To advance our understanding of RNA folding and dynamics it is critical to know the flexibility of these un-base-paired regions and how it depends on counterions. Yet, information about nucleic acid polymer properties is mainly derived from studies of ssDNA. Here we measure the persistence lengths (l(p)) of ssRNA. We observe valence and ionic strength-dependent differences in l(p) in a direct comparison between 40-mers of deoxythymidylate (dT(40)) and uridylate (rU(40)) measured using the powerful combination of SAXS and smFRET. We also show that nucleic acid flexibility is influenced by local environment (an adjoining double helix). Our results illustrate the complex interplay between conformation and ion environment that modulates nucleic acid function in vivo.
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