4.8 Article

AMP-activated protein kinase (AMPK) β1β2 muscle null mice reveal an essential role for AMPK in maintaining mitochondrial content and glucose uptake during exercise

出版社

NATL ACAD SCIENCES
DOI: 10.1073/pnas.1105062108

关键词

TBC1D1; PGC1-alpha; AS160; type 2 diabetes; obesity

资金

  1. National Health and Medical Research Council
  2. Australian Research Council
  3. Victorian Government
  4. Natural Sciences and Engineering Research Council
  5. Canadian Institutes of Health Research
  6. Melbourne University
  7. St. Vincent's Institute of Medical Research
  8. Canadian Diabetes Association
  9. McMaster University
  10. Danish Medical Research Council
  11. Lundbeck Research Foundation

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AMP-activated protein kinase (AMPK) beta 1 or beta 2 subunits are required for assembling of AMPK heterotrimers and are important for regulating enzyme activity and cellular localization. In skeletal muscle, alpha 2 beta 2 gamma 3-containing heterotrimers predominate. However, compensatory up-regulation and redundancy of AMPK subunits in whole-body AMPK alpha 2, beta 2, and gamma 3 null mice has made it difficult to determine the physiological importance of AMPK in regulating muscle metabolism, because these models have normal mitochondrial content, contraction-stimulated glucose uptake, and insulin sensitivity. In the current study, we generated mice lacking both AMPK beta 1 and beta 2 isoforms in skeletal muscle (beta 1 beta 2M-KO). beta 1 beta 2M-KO mice are physically inactive and have a drastically impaired capacity for treadmill running that is associated with reductions in skeletal muscle mitochondrial content but not a fiber-type switch. Interestingly, young beta 1 beta 2M-KO mice fed a control chow diet are not obese or insulin resistant but do have impaired contraction-stimulated glucose uptake. These data demonstrate an obligatory role for skeletal muscle AMPK in maintaining mitochondrial capacity and contraction-stimulated glucose uptake, findings that were not apparent in mice with single mutations or deletions in muscle alpha, beta, or gamma subunits.

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