期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 108, 期 6, 页码 2528-2533出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1019034108
关键词
optical imaging; prion-like transmission; seeding
资金
- National Institutes of Health [R37 AG031220]
- Hillblom Foundation
- Canadian Institutes of Heath Research (CIHR)
Transgenic (Tg) mouse models of Alzheimer's disease have served as valuable tools for investigating pathogenic mechanisms related to A beta accumulation. However, assessing disease status in these animals has required time-consuming behavioral assessments or postmortem neuropathological analysis. Here, we report a method for tracking the progression of A beta accumulation in vivo using bioluminescence imaging (BLI) on two lines of Tg mice, which express luciferase (luc) under control of the Gfap promoter as well as mutant human amyloid precursor protein. Bigenic mice exhibited an age-dependent increase in BLI signals that correlated with the deposition of A beta in the brain. Bioluminescence signals began to increase in 7-mo-old Tg(CRND8: Gfap-luc) mice and 14-mo-old Tg(APP23: Gfap-luc) mice. When Tg(APP23: Gfap-luc) mice were inoculated with brain homogenates from aged Tg(APP23) mice, BLI detected the accelerated disease onset and induced A beta deposition at 11 mo of age. Because of its rapid, noninvasive, and quantitative format, BLI permits the objective repeated analysis of individual mice at multiple time points, which is likely to facilitate the testing of A beta-directed therapeutics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据