期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 108, 期 48, 页码 19365-19370出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1108515108
关键词
structural biology; virology
资金
- Viral Hemorrhagic Fever Research Consortium
- National Institutes of Health [GM093325, GM020501, GM066170, NS070899, RR029388, AI077719, AI047140]
- Investigators in Pathogenesis of Infectious Diseases Award from the Burroughs Wellcome Fund
- Skaggs Institute for Chemical Biology
- [HHSN272200900049C]
- [BAA-NIAID-DAIT-NIHAI2008031]
Arenaviruses cause disease in industrialized and developing nations alike. Among them, the hemorrhagic fever virus Lassa is responsible for similar to 300,000-500,000 infections/y in Western Africa. The arenavirus nucleoprotein (NP) forms the protein scaffold of the genomic ribonucleoprotein complexes and is critical for transcription and replication of the viral genome. Here, we present crystal structures of the RNA-binding domain of Lassa virus NP in complex with ssRNA. This structure shows, in contrast to the predicted model, that RNA binds in a deep, basic crevice located entirely within the N-terminal domain. Furthermore, the NP-ssRNA structures presented here, combined with hydrogen-deuterium exchange/MS and functional studies, suggest a gating mechanism by which NP opens to accept RNA. Directed mutagenesis and functional studies provide a unique look into how the arenavirus NPs bind to and protect the viral genome and also suggest the likely assembly by which viral ribonucleoprotein complexes are organized.
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