期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 108, 期 21, 页码 E136-E144出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1103411108
关键词
gene movement; nuclear export
资金
- New England Regional Center of Excellence for Biodefense and Emerging Infectious Diseases (National Institutes of Health) [AI057159]
- National Institutes of Health [AI63106, R01GM080587]
- German Federal Ministry of Education and Research (BMBF) (FORSYS) [0313923]
The role of the intranuclear movement of chromatin in gene expression is not well-understood. Herpes simplex virus forms replication compartments (RCs) in infected cell nuclei as sites of viral DNA replication and late gene transcription. These structures develop from small compartments that grow in size, move, and coalesce. Quantitative analysis of RC trajectories, derived from 4D images, shows that most RCs move by directed motion. Directed movement is impaired in the presence of actin and myosin inhibitors as well as a transcription inhibitor. In addition, RCs coalesce at and reorganize nuclear speckles. Lastly, distinct effects of actin and myosin inhibitors on viral gene expression suggest that RC movement is not required for transcription, but rather, movement results in the bridging of transcriptionally active RCs with nuclear speckles to form structures that enhance export of viral late mRNAs.
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