期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 108, 期 27, 页码 10992-10996出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1104651108
关键词
cell size; apoptosis; hematology; biosensor; suspended microchannel resonator
资金
- EUREKA [R01GM085457]
- US National Institutes of Health [P50GM68762]
- US Army Research Office [W911NF-09-D-0001]
- Whitaker Health Sciences Graduate Fellowship
- National Institutes of Health [R01HL094270]
- National Institute of Diabetes and Digestive and Kidney Diseases [DK083242]
We have used a microfluidic mass sensor to measure the density of single living cells. By weighing each cell in two fluids of different densities, our technique measures the single-cell mass, volume, and density of approximately 500 cells per hour with a density precision of 0.001 gmL(-1). We observe that the intrinsic cell-to-cell variation in density is nearly 100-fold smaller than the mass or volume variation. As a result, we can measure changes in cell density indicative of cellular processes that would be otherwise undetectable by mass or volume measurements. Here, we demonstrate this with four examples: identifying Plasmodium falciparum malaria-infected erythrocytes in a culture, distinguishing transfused blood cells from a patient's own blood, identifying irreversibly sickled cells in a sickle cell patient, and identifying leukemia cells in the early stages of responding to a drug treatment. These demonstrations suggest that the ability to measure single-cell density will provide valuable insights into cell state for a wide range of biological processes.
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