期刊
EPIGENETICS & CHROMATIN
卷 8, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s13072-015-0023-7
关键词
Regulation of gene expression; Functional genomics; Stem cells; dCas9; CRISPR/Cas9; Zinc Finger; Transcription-Activator-Like Effector (TALE); Synthetic biology
资金
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- NSERC
Genome editing technology has evolved rather quickly and become accessible to most researchers. It has resulted in far reaching implications and a number of novel designer systems including epigenome editing. Epigenome editing utilizes a combination of nuclease-null genome editing systems and effector domains to modulate gene expression. In particular, Zinc Finger, Transcription-Activator-Like Effector, and CRISPR/Cas9 have emerged as modular systems that can be modified to allow for precision manipulation of epigenetic marks without altering underlying DNA sequence. This review contains a comprehensive catalog of effector domains that can be used with components of genome editing systems to achieve epigenome editing. Ultimately, the evidence-based design of epigenome editing offers a novel improvement to the limited attenuation strategies. There is much potential for editing and/or correcting gene expression in somatic cells toward a new era of functional genomics and personalized medicine.
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