期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 108, 期 32, 页码 13124-13129出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1100506108
关键词
quantitative phase imaging; population growth; adherent cell mass; label free imaging
资金
- National Science Foundation (NSF) [CBET-0939511, 08-46660, 0843604]
- Grainger Foundation
- National Institutes of Health [R01GM082837]
- Human Frontier Science Program [RGY 70/2008]
- NSF (Physics Frontiers Center: Center for the Physics of Living Cells) [082265]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0843604] Funding Source: National Science Foundation
Determining the growth patterns of single cells offers answers to some of the most elusive questions in contemporary cell biology: how cell growth is regulated and how cell size distributions are maintained. For example, a linear growth in time implies that there is no regulation required to maintain homeostasis; an exponential pattern indicates the opposite. Recently, there has been great effort to measure single cells using microelectromechanical systems technology, and several important questions have been explored. However, a unified, easy-to-use methodology to measure the growth rate of individual adherent cells of various sizes has been lacking. Here we demonstrate that a newly developed optical interferometric technique, known as spatial light interference microscopy, can measure the cell dry mass of many individual adherent cells in various conditions, over spatial scales from micrometers to millimeters, temporal scales ranging from seconds to days, and cell types ranging from bacteria to mammalian cells. We found evidence of exponential growth in Escherichia coli, which agrees very well with other recent reports. Perhaps most importantly, combining spatial light interference microscopy with fluorescence imaging provides a unique method for studying cell cycle-dependent growth. Thus, by using a fluorescent reporter for the S phase, we measured single cell growth over each phase of the cell cycle in human osteosarcoma U2OS cells and found that the G2 phase exhibits the highest growth rate, which is massdependent and can be approximated by an exponential.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据