期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 108, 期 49, 页码 19504-19509出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1109111108
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资金
- European doctoral school Frontieres du Vivant
- Fondation pour la Recherche Medicale
- Paris School of Neuroscience
- Centre National de la Recherche Scientifique
- Agence Nationale de la Recherche
- European Union Strep Photolysis [LSHM-CT-2007-037765]
- Human Frontier Science Program [RGP0013/2010]
- Fondation pour la Recherche Medicale (FRM equipe)
- Medical Research Council [U117512659] Funding Source: researchfish
Access to three-dimensional structures in the brain is fundamental to probe signal processing at multiple levels, from integration of synaptic inputs to network activity mapping. Here, we present an optical method for independent three-dimensional photoactivation and imaging by combination of digital holography with remote-focusing. We experimentally demonstrate compensation of spherical aberration for out-of-focus imaging in a range of at least 300 mu m, as well as scanless imaging along oblique planes. We apply this method to perform functional imaging along tilted dendrites of hippocampal pyramidal neurons in brain slices, after photostimulation by multiple spots glutamate uncaging. By bringing extended portions of tilted dendrites simultaneously in-focus, we monitor the spatial extent of dendritic calcium signals, showing a shift from a widespread to a spatially confined response upon blockage of voltage-gated Na+ channels.
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