期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 108, 期 50, 页码 20072-20077出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1111233109
关键词
Ca2+ signaling; phosphatidylinositol-3 phosphate; T helper cells; tripartite motif containing protein 27 knockout
资金
- National Institutes of Health [R01GM084195, R01AI052459]
The K+ channel KCa3.1 is required for Ca2+ influx and the subsequent activation of CD4 T cells. The class II phosphatidylinositol 3 kinase C2 beta (PI3KC2 beta) is activated by the T-cell receptor (TCR) and is critical for KCa3.1 channel activation. Tripartite motif containing protein 27 (TRIM27) is a member of a large family of proteins that function as Really Interesting New Gene (RING) E3 ubiquitin ligases. We now show that TRIM27 functions as an E3 ligase and mediates lysine 48 polyubiquitination of PI3KC2 beta, leading to a decrease in PI3K enzyme activity. By inhibiting PI3KC2 beta, TRIM27 also functions to negatively regulate CD4 T cells by inhibiting KCa3.1 channel activity and TCR-stimulated Ca2+ influx and cytokine production in Jurkat, primary human CD4 T cells, and Th0, Th1, and Th2 CD4 T cells generated from TRIM27(-/-) mice. These findings provide a unique mechanism for regulating class II PI3Ks, and identify TRIM27 as a previously undescribed negative regulator of CD4 T cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据