期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 108, 期 45, 页码 E1089-E1098出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1101494108
关键词
mitochondrion; longevity; Warburg effect
资金
- Ruth Kirschstein Cancer Training Grant [T32 CA-009528]
- National Institute of General Medical Sciences Center for Quantitative Biology [P50 GM-071508]
- National Institutes of Health [GM-046406]
Survival of yeast during starvation has been shown to depend on the nature of the missing nutrient(s). In general, starvation for natural nutrients such as sources of carbon, phosphate, nitrogen, or sulfate results in low death rates, whereas starvation for amino acids or other metabolites in auxotrophic mutants results in rapid loss of viability. Here we characterized phenotype, gene expression, and metabolite abundance during starvation for methionine. Some methionine auxotrophs (those with blocks in the biosynthetic pathway) respond to methionine starvation like yeast starving for natural nutrients such as phosphate or sulfate: they undergo a uniform cell cycle arrest, conserve glucose, and survive. In contrast, methionine auxotrophs with defects in the transcription factors Met31p and Met32p respond poorly, like other auxotrophs. We combined physiological and gene expression data from a variety of nutrient starvations (in both respiratory competent and incompetent cells) to show that successful starvation response is correlated with expression of genes encoding oxidative stress response and nonrespiratory mitochondrial functions, but not respiration per se.
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