期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 108, 期 25, 页码 10343-10348出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1105135108
关键词
-
资金
- European Community [22943]
- Swedish Research Council [K2007-62X-20391-01-4, K2007-62P-20390-01-4, K2010-80P-21583-01-4]
- Bagadilico Grant [349-2007-8626]
- Stem-Therapy
- Crafoord Foundation
- Swedish Parkinson Foundation
- Jeansson Foundation
- Lundbeck Foundation [R44-A3856]
- M. Lundqvist Foundation
- Knut and Alice Wallenberg Foundation
- Human Frontiers Science Program
- Lundbeck Foundation [R44-2009-3856] Funding Source: researchfish
Recent reports demonstrate that somatic mouse cells can be directly converted to other mature cell types by using combined expression of defined factors. Here we show that the same strategy can be applied to human embryonic and postnatal fibroblasts. By overexpression of the transcription factors Ascl1, Brn2, and Myt1l, human fibroblasts were efficiently converted to functional neurons. We also demonstrate that the converted neurons can be directed toward distinct functional neurotransmitter phenotypes when the appropriate transcriptional cues are provided together with the three conversion factors. By combining expression of the three conversion factors with expression of two genes involved in dopamine neuron generation, Lmx1a and FoxA2, we could direct the phenotype of the converted cells toward dopaminergic neurons. Such subtype-specific induced neurons derived from human somatic cells could be valuable for disease modeling and cell replacement therapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据