期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 107, 期 20, 页码 9452-9457出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1000675107
关键词
damage-associated molecular patterns; elongation factor tu receptor; pectin-mediated signaling; plant immunity; chimeric receptors
资金
- Ministero dell'Universita e della Ricerca
- ERA-NET Plant Genomics [RBER063SN4]
- European Reasearch Council (ERC) [233083]
- Ministero delle Politiche Agricole e Forestali
- Institute Pasteur-Fondazione Cenci Bolognetti
- Universita di Roma La Sapienza (ATE-NEO)
- European Research Council (ERC) [233083] Funding Source: European Research Council (ERC)
Oligogalacturonides (OGs) released from the plant cell wall are active both as damage-associated molecular patterns (DAMPs) for the activation of the plant immune response and regulators of plant growth and development. Members of the Wall-Associated Kinase (WAK) family are candidate receptors of OGs, due to their ability to bind in vitro these oligosaccharides. Because lethality and redundancy have hampered the study of WAKs by reverse genetics, we have adopted a chimeric receptor approach to elucidate the role of Arabidopsis WAK1. In a test-of-concept study, we first defined the appropriate chimera design and demonstrated that the Arabidopsis pattern recognition receptor (PRR) EFR is amenable to the construction of functional and resistance-conferring chimeric receptors carrying the ectodomain of another Arabidopsis PRR, FLS2. After, we analyzed chimeras derived from EFR and WAK1. Our results show that, upon stimulation with OGs, the WAK1 ectodomain is capable of activating the EFR kinase domain. On the other hand, upon stimulation with the cognate ligand elf18, the EFR ectodomain activates the WAK1 kinase, triggering defense responses that mirror those normally activated by OGs and are effective against fungal and bacterial pathogens. Finally, we show that transgenic plants overexpressing WAK1 are more resistant to Botrytis cinerea.
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