期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 107, 期 5, 页码 2060-2065出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0907471107
关键词
Bombyx mori; G protein-coupled receptor; neuropeptides; steroidogenesis
资金
- Enhancement of Center of Excellence
- JSPS [21380040, 19380034]
- Program for Promotion of Basic Research Activities for Innovative Biosciences
- Agentura na podporu vyskumu a vyvoja [APVV-51-039105]
- Vedecka grantova agentura [VEGA 2-6090-26]
- Natural Science Foundation of China
- University of Tokyo, Japan
- Grants-in-Aid for Scientific Research [21380040, 19380034] Funding Source: KAKEN
Insect molting and metamorphosis are induced by steroid hormones named ecdysteroids, whose production is regulated by various neuropeptides. We cloned the gene and analyzed the expression of the prothoracicostatic peptide, a unique neuropeptide shown to suppress the production of ecdysteroids in the prothoracic gland of the silkworm, Bombyx mori. We also characterized a Bombyx G protein-coupled receptor, which has previously been identified as an ortholog of the Drosophila sex peptide receptor, as a functional prothoracicostatic peptide receptor. This receptor responded specifically to the prothoracicostatic peptides when examined using a heterologous expression system. The receptor was highly expressed in the prothoracic gland on the day before each larval and pupal ecdysis, when prothoracicostatic peptides are synthesized at a high level in the epiproctodeal glands. These results suggest that the sex peptide receptor functions as a prothoracicostatic peptide receptor in Bombyx and that the peripheral neurosecretory cells as well as the central neuroendocrine system play stage-specific roles in regulating ecdysteroidogenesis.
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