期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 107, 期 24, 页码 10837-10841出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1005632107
关键词
biosensor; aptamer; visual detection; thrombin detection; cocaine detection
资金
- Heeger Presidential Chair Funds
- National Science Foundation under NSF-DMR [0602280]
- U.S. Army Research Office, Institute for Collaborative Biotechnologies [DAAD19-03-D-0004]
- National Institutes of Health [EB007689-02]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [0602280] Funding Source: National Science Foundation
We have demonstrated a novel sensing strategy employing single-stranded probe DNA, unmodified gold nanoparticles, and a positively charged, water-soluble conjugated polyelectrolyte to detect a broad range of targets including nucleic acid (DNA) sequences, proteins, small molecules, and inorganic ions. This nearly universal biosensor approach is based on the observation that, while the conjugated polyelectrolyte specifically inhibits the ability of single-stranded DNA to prevent the aggregation of gold-nanoparticles, no such inhibition is observed with double-stranded or otherwise folded DNA structures. Colorimetric assays employing this mechanism for the detection of hybridization are sensitive and convenient-picomolar concentrations of target DNA are readily detected with the naked eye, and the sensor works even when challenged with complex sample matrices such as blood serum. Likewise, by employing the binding-induced folding or association of aptamers we have generalized the approach to the specific and convenient detection of proteins, small molecules, and inorganic ions. Finally, this new biosensor approach is quite straightforward and can be completed in minutes without significant equipment or training overhead.
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