期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 107, 期 41, 页码 17833-17838出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0911359107
关键词
compartmentation; plant lipid metabolism
资金
- Office of Science (Biological and Environmental Research), US Department of Energy [DE-SC0000797]
- Natural Sciences and Engineering Research Council of Canada [217291]
- US Department of Agriculture-Agricultural Research Service-Current Research Information System [5347-21000-009]
- National Institutes of Health [HL-20948, GM-52016]
- Perot Family Foundation
- Cecil H. Green Distinguished Chair in Cellular and Molecular Biology
- Department of Biological Sciences
- Toulouse School of Graduate Studies (University of North Texas)
- U.S. Department of Energy (DOE) [DE-SC0000797] Funding Source: U.S. Department of Energy (DOE)
CGI-58 is the defective gene in the human neutral lipid storage disease called Chanarin-Dorfman syndrome. This disorder causes intracellular lipid droplets to accumulate in nonadipose tissues, such as skin and blood cells. Here, disruption of the homologous CGI-58 gene in Arabidopsis thaliana resulted in the accumulation of neutral lipid droplets in mature leaves. Mass spectroscopy of isolated lipid droplets from cgi-58 loss-of-function mutants showed they contain triacylglycerols with common leaf-specific fatty acids. Leaves of mature cgi-58 plants exhibited a marked increase in absolute triacylglycerol levels, more than 10-fold higher than in wild-type plants. Lipid levels in the oil-storing seeds of cgi-58 loss-of-function plants were unchanged, and unlike mutations in beta-oxidation, the cgi-58 seeds germinated and grew normally, requiring no rescue with sucrose. We conclude that the participation of CGI-58 in neutral lipid homeostasis of nonfat-storing tissues is similar, although not identical, between plant and animal species. This unique insight may have implications for designing a new generation of technologies that enhance the neutral lipid content and composition of crop plants.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据