期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 107, 期 38, 页码 16661-16666出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1007397107
关键词
silent synapse; synaptic development; synaptic plasticity; alpha bungarotoxin; cytisine
资金
- National Institutes of Health [NS45986]
- CHOP
In association with NMDA receptors (NMDARs), neuronal alpha 7 nicotinic ACh receptors (nAChRs) have been implicated in neuronal plasticity as well as neurodevelopmental, neurological, and psychiatric disorders. However, the role of presynaptic NMDARs and their interaction with alpha 7 nAChRs in these physiological and pathophysiological events remains unknown. Here we report that axonal alpha 7 nAChRs modulate presynaptic NMDAR expression and structural plasticity of glutamatergic presynaptic boutons during early synaptic development. Chronic inactivation of alpha 7 nAChRs markedly increased cell surface NMDAR expression as well as the number and size of glutamatergic axonal varicosities in cortical cultures. These boutons contained presynaptic NMDARs and alpha 7 nAChRs, and recordings from outside-out pulled patches of enlarged presynaptic boutons identified functional NMDAR-mediated currents. Multiphoton imaging of presynaptic NMDAR-mediated calcium transients demonstrated significantly larger responses in these enlarged boutons, suggesting enhanced presynaptic NMDAR function that could lead to increased glutamate release. Moreover, whole-cell patch clamp showed a significant increase in synaptic charge mediated by NMDAR miniature EPSCs but no alteration in the frequency of AMPAR miniature EPSCs, suggesting the selective enhancement of postsynaptically silent synapses upon inactivation of alpha 7 nAChRs. Taken together, these findings indicate that axonal alpha 7 nAChRs modulate presynaptic NMDAR expression and presynaptic and postsynaptic maturation of glutamatergic synapses, and implicate presynaptic alpha 7 nAChR/NMDAR interactions in synaptic development and plasticity.
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