期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 108, 期 3, 页码 1058-1063出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1016459108
关键词
Saccharomyces cerevisiae; sporulation; tiling arrays
资金
- Institut National de la Sante et de la Recherche Medicale (INSERM)/Institut National de l'Environnement Industriel et des Risques (INERIS)
- French Ministry of Education
- NSERM Avenir [R07216NS]
- Fondation pour la Recherche Medicale [INE20071111109]
- National Institutes of Health [5R01 GM068717, 5P01 HG000205]
Budding yeast noncoding RNAs (ncRNAs) are pervasively transcribed during mitosis, and some regulate mitotic protein-coding genes. However, little is known about ncRNA expression during meiotic development. Using high-resolution profiling we identified an extensive meiotic ncRNA expression program interlaced with the protein-coding transcriptome via sense/antisense transcript pairs, bidirectional promoters, and ncRNAs that overlap the regulatory regions of genes. Meiotic unannotated transcripts (MUTs) are mitotic targets of the conserved exosome component Rrp6, which itself is degraded after the onset of meiosis when MUTs and other ncRNAs accumulate in successive waves. Diploid cells lacking Rrp6 fail to initiate premeiotic DNA replication normally and cannot undergo efficient meiotic development. The present study demonstrates a unique function for budding yeast Rrp6 in degrading distinct classes of meiotically induced ncRNAs during vegetative growth and the onset of meiosis and thus points to a critical role of differential ncRNA expression in the execution of a conserved developmental program.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据