期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 107, 期 39, 页码 17023-17028出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1007773107
关键词
RNAi; post-transcriptional gene silencing; gluten intolerance; celiac sprue
资金
- Spanish Comision Interministerial de Ciencia y Tecnologia [AGL2007-65685-C02-01, AGL2010-19643-C02-02]
- European Regional Development Fund (ERDF)
- Norwegian Foundation for Health and Rehabilitation
- Novo Nordisk Foundation
- Consejo Superior de Investigaciones Cientificas
- European Social Fund
Celiac disease (CD) is an enteropathy triggered by the ingestion of gluten proteins from wheat and similar proteins from barley and rye. The inflammatory reaction is controlled by T cells that recognize gluten peptides in the context of human leukocyte antigen (HLA) DQ2 or HLA-DQ8 molecules. The only available treatment for the disease is a lifelong gluten-exclusion diet. We have used RNAi to down-regulate the expression of gliadins in bread wheat. A set of hairpin constructs were designed and expressed in the endosperm of bread wheat. The expression of gliadins was strongly down-regulated in the transgenic lines. Total gluten protein was extracted from transgenic lines and tested for ability to stimulate four different T-cell clones derived from the intestinal lesion of CD patients and specific for the DQ2-alpha-II, DQ2-gamma-VII, DQ8-alpha-I, and DQ8-gamma-I epitopes. For five of the transgenic lines, there was a 1.5-2 log reduction in the amount of the DQ2-alpha-II and DQ2-gamma-VII epitopes and at least 1 log reduction in the amount of the DQ8-alpha-I and DQ8-gamma-I epitopes. Furthermore, transgenic lines were also tested with two T-cell lines that are reactive with omega-gliadin epitopes. The total gluten extracts were unable to elicit T-cell responses for three of the transgenic wheat lines, and there were reduced responses for six of the transgenic lines. This work shows that the down-regulation of gliadins by RNAi can be used to obtain wheat lines with very low levels of toxicity for CD patients.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据