期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 106, 期 46, 页码 19322-19327出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0907001106
关键词
millisecond differential interference-contrast microscopy; Trypanosoma brucei; cilium; flagellum
资金
- National Science Foundation [DMR-0520894]
- National Institutes of Health/National Institute of Allergy and Infectious Diseases [AI052348]
- Alfred P. Sloan foundation
- Howard Hughes Medical Institute Gilliam fellowship
- Ruth L. Kirschstein National Research Service Award [GM07185]
- Burroughs Wellcome Fund
- University of California
Trypanosoma brucei, a parasitic protist with a single flagellum, is the causative agent of African sleeping sickness. Propulsion of T. brucei was long believed to be by a drill-like, helical motion. Using millisecond differential interference-contrast microscopy and analyzing image sequences of cultured procyclic-form and bloodstream-form parasites, as well as bloodstream-form cells in infected mouse blood, we find that, instead, motility of T. brucei is by the propagation of kinks, separating left-handed and right-handed helical waves. Kink-driven motility, previously encountered in prokaryotes, permits T. brucei a helical propagation mechanism while avoiding the large viscous drag associated with a net rotation of the broad end of its tapering body. Our study demonstrates that millisecond differential interference-contrast microscopy can be a useful tool for uncovering important short-time features of microorganism locomotion.
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