期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 106, 期 43, 页码 18357-18361出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0902573106
关键词
endothelial cell; polymerase II; RNA; tumor necrosis factor alpha
资金
- Special Coordination Fund for Promoting Science and Technology
- New Energy and Industrial Technology Development Organization
- National Institute of Biomedical Innovation of Japan
- Strategic Information and Communications R&D Promotion Program
- Ministry of Internal Affairs and Communications
- Genome Network Project, Japan [16101006, 19800009, 19310129]
- Ministry of Education, Culture, Sports, Science and Technology, and the Japan Society
- Grants-in-Aid for Scientific Research [20221010, 16101006, 19800009, 19310129] Funding Source: KAKEN
Genome-wide studies reveal that transcription by RNA polymerase II (Pol II) is dynamically regulated. To obtain a comprehensive view of a single transcription cycle, we switched on transcription of five long human genes (>100 kbp) with tumor necrosis factor-alpha (TNF alpha) and monitored (using microarrays, RNA fluorescence in situ hybridization, and chromatin immunoprecipitation) the appearance of nascent RNA, changes in binding of Pol II and two insulators (the cohesin subunit RAD21 and the CCCTC-binding factor CTCF), and modifications of histone H3. Activation triggers a wave of transcription that sweeps along the genes at approximate to 3.1 kbp/min; splicing occurs cotranscriptionally, a major checkpoint acts several kilobases downstream of the transcription start site to regulate polymerase transit, and Pol II tends to stall at cohesin/CTCF binding sites.
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