期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 106, 期 7, 页码 2159-2164出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0809567106
关键词
cellular imaging; molecular labels
资金
- Laboratory Directed Research and Development Program of Oak Ridge National Laboratory
- U. S. Department of Energy [DE-AC05-00OR22725]
- Shared Research Equipment ( SHARE)
- Vanderbilt University Medical Center
- National Institutes of Health [R01-RR018470, P20-GM072048]
Single gold-tagged epidermal growth factor (EGF) molecules bound to cellular EGF receptors of fixed fibroblast cells were imaged in liquid with a scanning transmission electron microscope (STEM). The cells were placed in buffer solution in a microfluidic device with electron transparent windows inside the vacuum of the electron microscope. A spatial resolution of 4 nm and a pixel dwell time of 20 mu s were obtained. The liquid layer was sufficiently thick to contain the cells with a thickness of 7 +/- 1 mu m. The experimental findings are consistent with a theoretical calculation. Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods.
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